Magnetically responsive microflaps reveal cell membrane boundaries from multiple angles

Tetsuhiko Teshima; Hiroaki Onoe; Hiroka Aonuma; Kaori Kuribayashi-Shigetomi; Koki Kamiya; Taishi Tonooka; Hirotaka Kanuka; Shoji Takeuchi

doi:10.1002/adma.201305494

Magnetically responsive microflaps reveal cell membrane boundaries from multiple angles

Adv Mater. 2014 May;26(18):2850-6. doi: 10.1002/adma.201305494. Epub 2014 Feb 22.

Authors

Tetsuhiko Teshima¹, Hiroaki Onoe, Hiroka Aonuma, Kaori Kuribayashi-Shigetomi, Koki Kamiya, Taishi Tonooka, Hirotaka Kanuka, Shoji Takeuchi

Affiliation

¹ Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8505, Japan.

PMID: 24677083
DOI: 10.1002/adma.201305494

Abstract

A microflap system to incline adherent cells in the desired orientation is described. Inclination angles of cell-laden microflaps are precisely controlled by the applied magnetic field, enabling us to observe cell-membrane boundaries from multiple angles. This system is equipped with conventional microscopes, allowing clear focused images of cell-membrane boundaries to be obtained with high magnification.

Keywords: magnetic actuation; multi-angle observation; parasite infection.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Membrane / ultrastructure*
Cell Polarity
Cells, Cultured
Equipment Design
Equipment Failure Analysis
Fibroblasts / cytology*
Humans
Image Enhancement / instrumentation*
Magnetics / instrumentation*
Micromanipulation / instrumentation*
Micromanipulation / methods
Microscopy / instrumentation*
Miniaturization