Effect of lanthanum(III) on the production of ethylene and reactive oxygen species in soybean seedlings exposed to the enhanced ultraviolet-B radiation

Qing Yang; Yueli Li; Lihong Wang; Qing Zhou; Xiaohua Huang

doi:10.1016/j.ecoenv.2014.02.026

Effect of lanthanum(III) on the production of ethylene and reactive oxygen species in soybean seedlings exposed to the enhanced ultraviolet-B radiation

Ecotoxicol Environ Saf. 2014 Jun:104:152-9. doi: 10.1016/j.ecoenv.2014.02.026. Epub 2014 Mar 25.

Authors

Qing Yang¹, Yueli Li¹, Lihong Wang¹, Qing Zhou², Xiaohua Huang³

Affiliations

¹ State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; School of Environment and Civil Engineering, Jiangnan University, Wuxi 214122, China.
² State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; School of Environment and Civil Engineering, Jiangnan University, Wuxi 214122, China. Electronic address: qingzhou510@yahoo.com.
³ Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, Jiangsu Key Laboratory of Biomedical Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210046, China; School of Environment and Civil Engineering, Jiangnan University, Wuxi 214122, China. Electronic address: huangxiaohuanjnu@yahoo.com.

PMID: 24675444
DOI: 10.1016/j.ecoenv.2014.02.026

Abstract

The enhanced ultraviolet-B (UV-B) radiation caused by ozone depletion may exert deleterious effects on plants. Therefore, studies on the effect of UV-B radiation on plants, as well as studies on the methods for alleviating the deleterious effects by chemical control, are of great significance. In this study, after soybean (Glycine max) seedlings were exposed to UV-B radiation (10.2 and 13.8kJ m(-2)day(-1)) for 5 days and the followed 6 days of restoration, respectively, the effects of 20mg L(-1) lanthanum (III) [La(III)] on leaf phenotype, photosynthetic rate, and production of ethylene and reactive oxygen species (ROS) were investigated. The results indicated that the exposure to 10.2 and 13.8kJ m(-2)day(-1) UV-B radiation could cause injury to the leaf phenotype, and lead to the decrease in the content of chlorophyll and the net photosynthetic rate, and the increase in the contents of ROS, ethylene and 1-aminocyclopropanecarboxylic acid, and 1-aminocyclopropanecarboxylic acid synthase activity in soybean seedlings. Following the withdrawal of the enhanced UV-B radiation, the above mentioned parameters gradually recovered, and the recovery of soybean seedlings exposed to 10.2kJ m(-2)day(-1) UV-B radiation was faster than those in soybean seedlings exposed to 13.8kJ m(-2)day(-1) UV-B radiation. The leaf injury and the changes in the above indices that were induced by the enhanced UV-B radiation, especially at 10.2kJ m(-2)day(-1), were alleviated after the pretreatment of soybean seedlings with 20mg L(-1) La(III). The results of the correlation analysis demonstrated that the injury to the leaf phenotype and the decrease in the photosynthetic rate of soybean seedlings were correlated with the increase in the ROS content that was induced by ethylene in soybean seedlings. The pretreatment with 20mg L(-1) La(III) alleviated the injury caused by the enhanced UV-B radiation through the regulation of the ROS production.

Keywords: Enhanced UV-B radiation; Lanthanum; Reactive oxygen species; Soybean seedling; Stress ethylene.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Ethylenes / metabolism*
Glycine max / drug effects*
Glycine max / radiation effects*
Lanthanum / pharmacology*
Photosynthesis / drug effects
Photosynthesis / radiation effects
Plant Leaves / drug effects
Plant Leaves / radiation effects
Reactive Oxygen Species / metabolism*
Seedlings / drug effects
Seedlings / radiation effects
Ultraviolet Rays*

Substances

Ethylenes
Reactive Oxygen Species
Lanthanum
ethylene