Electron spin resonance spectroscopy for the study of nanomaterial-mediated generation of reactive oxygen species

Weiwei He; Yitong Liu; Wayne G Wamer; Jun-Jie Yin

doi:10.1016/j.jfda.2014.01.004

Electron spin resonance spectroscopy for the study of nanomaterial-mediated generation of reactive oxygen species

J Food Drug Anal. 2014 Mar;22(1):49-63. doi: 10.1016/j.jfda.2014.01.004. Epub 2014 Feb 1.

Authors

Weiwei He¹, Yitong Liu², Wayne G Wamer², Jun-Jie Yin³

Affiliations

¹ Key Laboratory of Micro-Nano Materials for Energy Storage and Conversion of Henan Province, Institute of Surface Micro and Nano Materials, Xuchang University, Xuchang, Henan, China; Center for Food Safety and Applied Nutrition, US Food and Drug Administration, College Park, MD 20740, USA.
² Center for Food Safety and Applied Nutrition, US Food and Drug Administration, College Park, MD 20740, USA.
³ Center for Food Safety and Applied Nutrition, US Food and Drug Administration, College Park, MD 20740, USA. Electronic address: junjie.yin@fda.hhs.gov.

Abstract

Many of the biological applications and effects of nanomaterials are attributed to their ability to facilitate the generation of reactive oxygen species (ROS). Electron spin resonance (ESR) spectroscopy is a direct and reliable method to identify and quantify free radicals in both chemical and biological environments. In this review, we discuss the use of ESR spectroscopy to study ROS generation mediated by nanomaterials, which have various applications in biological, chemical, and materials science. In addition to introducing the theory of ESR, we present some modifications of the method such as spin trapping and spin labeling, which ultimately aid in the detection of short-lived free radicals. The capability of metal nanoparticles in mediating ROS generation and the related mechanisms are also presented.

Keywords: Electron spin resonance; Lipid peroxidation; Nanomaterials; Reactive oxygen species.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.
Review

MeSH terms

Electron Spin Resonance Spectroscopy* / methods
Humans
Nanostructures / analysis
Nanostructures / chemistry*
Reactive Oxygen Species / analysis
Reactive Oxygen Species / chemistry*

Substances

Reactive Oxygen Species

Grants and funding

W.H. also acknowledges the support from the National Natural Science Foundation of China (grant no. 21303153).