Detection of high-risk human papillomavirus type 16 and 18 using isothermal helicase-dependent amplification

Daniela Barbieri; Simona Venturoli; Frank Rösl; Bladimiro Rincon-Orozco

doi:10.1016/j.diagmicrobio.2014.02.012

Detection of high-risk human papillomavirus type 16 and 18 using isothermal helicase-dependent amplification

Diagn Microbiol Infect Dis. 2014 Jun;79(2):178-82. doi: 10.1016/j.diagmicrobio.2014.02.012. Epub 2014 Feb 24.

Authors

Daniela Barbieri¹, Simona Venturoli², Frank Rösl³, Bladimiro Rincon-Orozco⁴

Affiliations

¹ Department of Haematology, Oncology and Laboratory Medicine, Unit of Microbiology, University Hospital S. Orsola-Malpighi, University of Bologna, via Massarenti 9, 40138 Bologna, Italy; Division of Viral Transformation Mechanisms, German Cancer Research Center, Im Neuenheimer Feld 242, D-69120 Heidelberg, Germany.
² Department of Haematology, Oncology and Laboratory Medicine, Unit of Microbiology, University Hospital S. Orsola-Malpighi, University of Bologna, via Massarenti 9, 40138 Bologna, Italy.
³ Division of Viral Transformation Mechanisms, German Cancer Research Center, Im Neuenheimer Feld 242, D-69120 Heidelberg, Germany.
⁴ Division of Viral Transformation Mechanisms, German Cancer Research Center, Im Neuenheimer Feld 242, D-69120 Heidelberg, Germany. Electronic address: b.orozco@dkfz-heidelberg.de.

PMID: 24666707
DOI: 10.1016/j.diagmicrobio.2014.02.012

Abstract

Persistent infection with human papillomavirus (HPV) induces cervical cancer. Here, we describe a sensitive, specific, and rapid assay for high-risk HPV16 and 18 detection by isothermal helicase-dependent amplification. This method can be used as cost-effective diagnostic method for low-income countries, where highest incidences worldwide of cervical cancer are registered.

Keywords: Cervical cancer; Cost-effective method; HPV detection; Isothermal helicase-dependent amplification.

Publication types

Evaluation Study

MeSH terms

Female
Human papillomavirus 16 / genetics
Human papillomavirus 16 / isolation & purification*
Human papillomavirus 18 / genetics
Human papillomavirus 18 / isolation & purification*
Humans
Mass Screening / methods
Molecular Diagnostic Techniques / methods*
Nucleic Acid Amplification Techniques / methods*
Papillomavirus Infections / diagnosis*
Papillomavirus Infections / virology*
Sensitivity and Specificity
Temperature