FilmArray® respiratory panel performance in respiratory samples from neonatal care units

Diagn Microbiol Infect Dis. 2014 Jun;79(2):183-6. doi: 10.1016/j.diagmicrobio.2014.02.010. Epub 2014 Feb 24.

Abstract

FilmArray Respiratory Panel (RP) (Idaho Technology, Inc., Salt Lake City, UT, USA) performance was retrospectively evaluated in respiratory samples collected from neonates in 2 reference neonatology units. Using the FilmArray RP assay, 121/152 (79.6%) samples were positive for at least 1 respiratory virus, while 31/152 (20.4%) were negative. FilmArray RP results were concordant in 68/72 (94.4%) respiratory samples tested with laboratory-developed real-time PCR assays, while in 4/72 (5.6%) samples, the FilmArray RP assay detected an additional virus (2 human rhinovirus/enterovirus and 2 bocavirus). In addition, FilmArray RP results for 70 of 80 (87.5%) respiratory samples tested were concordant with the Seegene Seeplex RV15® detection assay (Seegene, Inc., Seoul, South Korea), while 10/80 (12.5%) were discordant. The advantages of the FilmArray RP are the rapid detection of respiratory viruses (1 hour), the wide number of pathogens detectable in a single assay, and the reduced hands-on time.

Keywords: FilmArray; Multiple PCR tests; Respiratory viruses.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Humans
  • Infant, Newborn
  • Molecular Diagnostic Techniques / methods*
  • Respiratory Tract Infections / diagnosis*
  • Respiratory Tract Infections / virology
  • Retrospective Studies
  • Sensitivity and Specificity
  • Time Factors
  • Virus Diseases / diagnosis*
  • Virus Diseases / virology