Objective: To study the biological characteristics of p75 neurotrophin receptor positive (p75(NTR+)) tongue squamous cell carcinoma cells which were separated by flow cytometry cell sorting.
Methods: To determine the biological characteristics of p75(NTR+) cells which were separated from Tca-8113 and Cal-27 tongue squamous cell carcinoma cells by flow cytometry cell sorting, including study the capacity of cloning, 3-(4,5)-demethylthiazo(z-y1)-3,5-diphenytetrazoliumromide (MTT) assay, wound healing assay. p75(NTR+) cells with non-sorted cells were as control group.
Results: In Tca-8113 and Cal-27 tongue squamous cell carcinoma cell lines, the percentage of p75(NTR+) cells were 3.1% and 1.9%. Compared with p75(NTR+) cells with non-sorted cells, p75(NTR+) cells possess higher capacity of cloning (Tca-8113, P=0.024; Cal-27, P=0.009). The percentage of p75(NTR+) cells of the progeny cells generated from monoclonal p75(NTR+) cells decreased to 14.5% (Tca-8113) and 5.8% (Cal-27) after cultured two weeks. p75(NTR+) cells possessed higher proliferation ability and higher metastasis ability than non-sorted cells.
Conclusion: p75(NTR+) cells isolated from tongue squamous cell carcinoma have the characteristics of cancer stem cells.
目的: 对流式细胞仪分选获得的p75神经营养蛋白受体（p75NTR）阳性舌鳞状细胞癌细胞进行生物学特性研究。
方法: 通过流式细胞术检测舌鳞状细胞癌细胞系Tca-8113和Cal-27中p75NTR阳性细胞的表达。对流式细胞仪分选获取的p75NTR阳性细胞进行单克隆培养、四甲基偶氮唑盐比色法及划痕实验检测，以未分选细胞为对照，分析p75NTR阳性细胞的生物学特性。
结果: 舌鳞状细胞癌细胞株Tca-8113、Cal-27中，p75NTR阳性细胞的比例分别为3.1％和1.9％。与未分选的细胞相比，p75NTR阳性细胞的单克隆形成能力更高（Tca-8113细胞，P=0.024；Cal-27细胞，P=0.009）；单克隆p75NTR阳性细胞再培养2周后，p75NTR阳性细胞率分别降为14.5%（Tca-8113）和5.8%（Cal-27）；p75NTR阳性细胞体外增殖能力显著增强，且具有明显的侵袭迁移能力。
结论: p75NTR阳性舌鳞状细胞癌细胞具有肿瘤干细胞的特性。