Epithelial cells form organized sheets to protect underlying tissues and maintain the physiological environment by the assembly of tight junctions (TJs) and adherens junctions (AJs), which mainly regulate paracellular molecular passage and selective cell-cell adhesion, respectively. At the cytoplasmic surface, TJs and AJs associate with a specific actomyosin cytoskeletal structure called the perijunctional actomyosin ring (PJAR), which encircles cells in a belt-like manner. ZO family proteins play important roles in regulating TJ and PJAR organization. We recently found that ARHGEF11, a member of the RGS-RhoGEF family of proteins, associates with TJs by binding to ZO-1. ARHGEF11 mediates ZO-1-dependent junction assembly and barrier formation in mammary epithelial cells. Another recent study demonstrated that ARHGEF11-dependent apical actomyosin contraction is coupled to planar cell polarity signaling in neuroepithelial cells for the control of neural tube formation. These findings suggest that ARHGEF11 generally regulates apical junctions and junction-associated actomyosin in various epithelial tissues.
Keywords: ARHGEF11; Rho signaling; RhoGEF; ZO-1; epithelial barrier; perijunctional actomyosin ring; tight junction.