Maputaland-Pondoland-Albany, South Africa has been identified as a biodiversity hotspot and centre for endemism. Odonata make good indicators of freshwater ecosystem health. Consequently we compiled a list of Odonata species recorded to date in the iSimangaliso Wetland Park. We then detailed important species in terms of endemism, conservation status, and potential as indicator species. Finally, we compared Odonata assemblages of different sites sampled within the park to illustrate habitat importance. Species identified during two formal surveys and incidental observations made during the study period were combined with an existing database to compile an accurate and up to date species list for the iSimangaliso Wetland Park. Data from this study were then analyzed to determine which water bodies had the most similar species composition. The Dragonfly Biotic Index (DBI) value of each study area was also determined. We recorded 68 odonate species in the iSimangaliso Wetland Park, adding 13 species to the Ezemvelo KwaZulu-Natal Wildlife database for the area. This brings the total number of Odonata species for the iSimangaliso Wetland Park to 86. Eight species are red-listed, 12 are restricted in South Africa to the coastal plains of northern KwaZulu-Natal, and the remainder occurs widely across the southern African savanna. Analyses indicate that species odonate assemblages were most similar in water bodies with comparable habitats. iSimangaliso Wetland Park is identified as an important area for Odonata diversity and endemism, a trend also reflected by the DBI values. Shifts in the existing species assemblages would indicate changes within the ecosystem and thus this species account provides necessary baseline data for the area. Species Conservation efforts should thus target water bodies of varying habitat types to protect greater species diversity.