A density gradient of basic fibroblast growth factor guides directional migration of vascular smooth muscle cells

Jindan Wu; Zhengwei Mao; Lulu Han; Yizhi Zhao; Jiabin Xi; Changyou Gao

doi:10.1016/j.colsurfb.2014.02.043

A density gradient of basic fibroblast growth factor guides directional migration of vascular smooth muscle cells

Colloids Surf B Biointerfaces. 2014 May 1:117:290-5. doi: 10.1016/j.colsurfb.2014.02.043. Epub 2014 Mar 2.

Authors

Jindan Wu¹, Zhengwei Mao², Lulu Han², Yizhi Zhao², Jiabin Xi², Changyou Gao³

Affiliations

¹ MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China; MOE Key Laboratory of Advanced Textile Materials & Manufacturing Technology, College of Materials and Textile, Zhejiang Sci-Tech University, Hangzhou 310018, China.
² MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
³ MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China. Electronic address: cygao@mail.hz.zj.cn.

PMID: 24657928
DOI: 10.1016/j.colsurfb.2014.02.043

Abstract

The migration of vascular smooth muscle cells (VSMCs) is an important process in many physiological events. It is of paramount importance to control the migration rate and direction of VSMCs by biomaterials. In this paper, a density gradient of basic fibroblast growth factor (bFGF) was fabricated using an injection method and the bio-conjugation between heparin and bFGF. The density of bFGF gradually increased with a slope of 17 ng/cm(2)/mm. Adhesion and migration of VSMCs were studied on the bFGF gradient. The VSMCs exhibited preferential orientation and an enhanced directional migration behavior on the gradient surface. Up to 70% cells migrated towards the region with a higher density of bFGF on the gradient. However, the bFGF gradient had no effect on the cell migration rate.

Keywords: Basic fibroblast growth factor; Biomaterials; Cell migration; Gradient; Smooth muscle cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Adhesion / drug effects
Cell Movement / drug effects*
Fibroblast Growth Factor 2 / pharmacology*
Fluorescence
Humans
Immunohistochemistry
Muscle, Smooth, Vascular / cytology*
Myocytes, Smooth Muscle / cytology*
Myocytes, Smooth Muscle / drug effects

Substances

Fibroblast Growth Factor 2