Influence of surface-imprinted nanoparticles on trypsin activity

Abstract

Here, the modulation of enzyme activity is presented by protein-imprinted nanoparticles produced using a solid-phase approach. Using trypsin as target, binding of the nanoparticles to the enzyme results in its inhibition or in stabilization, depending on the orientation of the immobilized enzyme used during imprinting.

Keywords: enzyme inhibition; molecular recognition; molecularly imprinted polymers; nanoparticles; supramolecular chemistry.