Therapeutic interaction of systemically-administered mesenchymal stem cells with peri-implant mucosa

PLoS One. 2014 Mar 20;9(3):e90681. doi: 10.1371/journal.pone.0090681. eCollection 2014.

Abstract

Objectives: The objective of this study was to investigate the effect of systemically transplanted mesenchymal stem cells (MSCs) on the peri-implant epithelial sealing around dental implants.

Materials and methods: MSCs were isolated from bone marrow of donor rats and expanded in culture. After recipient rats received experimental titanium dental implants in the bone sockets after extraction of maxillary right first molars, donor rat MSCs were intravenously transplanted into the recipient rats.

Results: The injected MSCs were found in the oral mucosa surrounding the dental implants at 24 hours post-transplantation. MSC transplantation accelerated the formation of the peri-implant epithelium (PIE)-mediated mucosa sealing around the implants at an early stage after implantation. Subsequently, enhanced deposition of laminin-332 was found along the PIE-implant interface at 4 weeks after the replacement. We also observed enhanced attachment and proliferation of oral mucous epithelial cells.

Conclusion: Systemically transplanted MSCs might play a critical role in reinforcing the epithelial sealing around dental implants.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bone Marrow Cells / cytology
  • Cell Adhesion Molecules
  • Cell Differentiation
  • Cell Separation
  • Coculture Techniques
  • Dental Implants*
  • Epithelial Cells / cytology
  • Green Fluorescent Proteins / metabolism
  • Kalinin
  • Male
  • Mesenchymal Stem Cell Transplantation*
  • Mesenchymal Stem Cells / cytology*
  • Mouth Mucosa / cytology*
  • Multipotent Stem Cells / cytology
  • Rats, Transgenic
  • Rats, Wistar
  • Tooth Extraction
  • Wound Healing

Substances

  • Cell Adhesion Molecules
  • Dental Implants
  • Green Fluorescent Proteins

Grants and funding

This work was supported by a grant-in-aid for Scientific Research (C) No. 23592888 (to I. Atsuta) from the Ministry of Education, Culture, Sports, Science and Technology of Japan. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.