Abstract
Bioactivity-guided isolation of the EtOAc extract of the stems of Millettia dielsiana Harms yielded two new isoflavones together with nine known ones. Their structures were elucidated by analysis of the spectroscopic data including 2D NMR. All of the isolates were evaluated for their potential to inhibit the LPS-induced production of nitric oxide and TNF-α in murine macrophage RAW 264.7 cells. Among the tested compounds, Millesianin C (1) had the most potent anti-inflammatory effect decreasing NO production similar to that of dexamethasone and decreasing TNF-α secretion better than that of dexamethasone. Their structure-activity relationship was also analyzed.
Keywords:
Anti-inflammation; Millettia dielsiana Harms; NO; SAR; TNF-α.
Copyright © 2014 Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Anti-Inflammatory Agents / chemistry
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Anti-Inflammatory Agents / isolation & purification*
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Anti-Inflammatory Agents / pharmacology
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Dexamethasone / pharmacology
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Flavonoids / chemistry
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Flavonoids / isolation & purification*
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Flavonoids / pharmacology
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Isoflavones / chemistry
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Isoflavones / isolation & purification*
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Isoflavones / pharmacology
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Lipopolysaccharides / metabolism
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Macrophages / drug effects
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Magnetic Resonance Spectroscopy
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Mass Spectrometry
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Medicine, Chinese Traditional
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Mice
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Millettia / chemistry*
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Molecular Structure
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Nitric Oxide / analysis
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Nitric Oxide / metabolism*
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Plant Stems / chemistry
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Plants, Medicinal
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Structure-Activity Relationship
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Tumor Necrosis Factor-alpha / drug effects
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Tumor Necrosis Factor-alpha / metabolism
Substances
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Anti-Inflammatory Agents
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Flavonoids
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Isoflavones
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Lipopolysaccharides
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Tumor Necrosis Factor-alpha
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millesianin H
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millesianin I
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Nitric Oxide
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Dexamethasone