Circular permutation of chicken interleukin-1 beta enhances its thermostability

Chem Commun (Camb). 2014 Apr 25;50(32):4248-50. doi: 10.1039/c3cc48313d.

Abstract

Interleukin-1β is a cytokine critically involved in immune and inflammatory responses. To extend its use as a component of avian vaccines, a circularly permuted chicken interleukin-1β was synthesized that maintains its activity after pre-incubation at high temperatures, unlike wild-type chicken interleukin-1β, which is irreversibly inactivated at high temperatures.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chickens / immunology
  • Chickens / metabolism*
  • Circular Dichroism
  • Fluorescence
  • Hot Temperature*
  • Interleukin-1beta / chemistry*
  • Interleukin-1beta / genetics
  • Interleukin-1beta / metabolism
  • Mutation / genetics
  • Protein Stability*
  • Receptors, Interleukin-1 / metabolism
  • Surface Plasmon Resonance

Substances

  • Interleukin-1beta
  • Receptors, Interleukin-1