Novel expansins, non-catalytic proteins which induce weakening of the rigid cellulose structure, have been identified in this study. A pipeline of bioinformatics was implemented for sequence and structure-based prediction of putative bacterial expansin-like group × family from NR databases. All putative expansins had no detectable activity against cellulosic and hemicellulosic substrates but showed varying degrees of synergy (2.0-7.6 folds) with the commercial Trichoderma reesei cellulase (Celluclast™ 1.5L) on degradation of filter paper in order of BpEX ≈ CmEX > MaEX > PcEX > SaEX. A mixture design with full cubic model predicted optimal formulation comprising Celluclast™: CmEX from Clavibacter michiganensis = 72.4%: 27.6%, with no synergy of β-glucosidase on degradation of alkaline pretreated rice straw. Under these conditions, the reducing sugar yield was 163.6% compared with the reaction containing cellulase alone. This work demonstrated the potential benefit of novel bacterial expansins on enhancing cellulose degradation efficiency in lignocellulosic biomass degradation.
Keywords: Cellulase; Expansin; Lignocellulose; Mixture design; Synergy.
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