CYP1A1 gene polymorphisms and smoking status as modifier factors for lung cancer risk

Atef G Hussein; Heba F Pasha; Hanan M El-Shahat; Doaa M Gad; Mostafa M Toam

doi:10.1016/j.gene.2014.03.003

CYP1A1 gene polymorphisms and smoking status as modifier factors for lung cancer risk

Gene. 2014 May 10;541(1):26-30. doi: 10.1016/j.gene.2014.03.003. Epub 2014 Mar 5.

Authors

Atef G Hussein¹, Heba F Pasha², Hanan M El-Shahat³, Doaa M Gad³, Mostafa M Toam⁴

Affiliations

¹ Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
² Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt. Electronic address: hebapasha@yahoo.com.
³ Chest Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
⁴ Clinical Oncology and Nuclear Medicine Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.

PMID: 24613751
DOI: 10.1016/j.gene.2014.03.003

Abstract

Objective: Lung cancer remains the most prevalent malignancy worldwide. Susceptibility to lung cancer has been shown to be modulated by inheritance of polymorphic genes. Several metabolic enzymes are currently under investigation for their possible role in lung cancer susceptibility, including members of the cytochrome P450 (CYP) superfamily. The aim of this work was to identify the correlation between CYP1A1 m1 and m2 polymorphisms and lung cancer risk and figure its interactions with smoking as genetic modifiers in the etiology of lung cancer in the Egyptian population.

Materials and methods: One hundred and ten patients with lung cancer and one hundred and ten controls were enrolled in the study. CYP1A1 m1 and m2 polymorphisms were determined using polymerase chain reaction restriction fragment length polymorphism.

Results: Subjects carrying TC and CC genotypes of CYP1A1 m1 and AG and GG genotypes of CYP1A1 m2 were significantly more likely to develop lung cancer especially squamous cell carcinoma. The proportion of lung cancer attributable to the interaction of smoking and CYP1A1 m1 and CYP1A1 m2 polymorphisms was 32% and 52% respectively.

Conclusion: Our results revealed that CYP1A1 m1 and m2 polymorphisms contribute to smoking related lung cancer risk in the Egyptian population.

Keywords: CYP1A1 m1; CYP1A1 m2; Lung cancer; Polymorphism.

MeSH terms

Aged
Alleles
Carcinoma, Squamous Cell / genetics
Case-Control Studies
Cytochrome P-450 CYP1A1 / genetics*
Egypt
Female
Genetic Predisposition to Disease*
Genotype
Humans
Lung Neoplasms / ethnology
Lung Neoplasms / genetics*
Lung Neoplasms / metabolism
Male
Middle Aged
Polymorphism, Genetic*
Polymorphism, Restriction Fragment Length
Risk Factors
Sequence Analysis, DNA
Smoking*

Substances

CYP1A1 protein, human
Cytochrome P-450 CYP1A1