Optimization on preparation conditions of Rehmannia glutinosa polysaccharide liposome and its immunological activity

Yee Huang; Caihong Wu; Zhenguang Liu; Yuanliang Hu; Cheng Shi; Yun Yu; Xiaojuan Zhao; Cui Liu; Jiaguo Liu; Yi Wu; Deyun Wang

doi:10.1016/j.carbpol.2014.01.022

Optimization on preparation conditions of Rehmannia glutinosa polysaccharide liposome and its immunological activity

Carbohydr Polym. 2014 Apr 15:104:118-26. doi: 10.1016/j.carbpol.2014.01.022. Epub 2014 Jan 17.

Authors

Yee Huang¹, Caihong Wu², Zhenguang Liu¹, Yuanliang Hu¹, Cheng Shi¹, Yun Yu¹, Xiaojuan Zhao¹, Cui Liu¹, Jiaguo Liu¹, Yi Wu¹, Deyun Wang³

Affiliations

¹ Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
² Jiangsu Animal Husbandry & Veterinary College, Taizhou, Jiangsu Province 225300, PR China.
³ Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China. Electronic address: dywang@njau.edu.cn.

PMID: 24607168
DOI: 10.1016/j.carbpol.2014.01.022

Abstract

The aim of this study was to investigate the optimizing preparation conditions and immunological enhancement activity of Rehmannia glutinosa polysaccharide liposome (RGPL). RGPL was prepared using the reverse-phase evaporation method and optimized using the response surface methodology. The immunological enhancement activity of RGPL on splenocyte proliferation was measured. These results showed that the optimum preparation conditions were: a soybean phosphatide to cholesterol ratio of 8:1, a chloroform to methanol ratio of 3:5, a soybean phosphatide to tween 80 ratio of 10:1, a temperature (°C) of 66°C and an entrapment efficiency of 72.753 ± 0.318%. In a single stimulation of drugs, RGPL could significantly promote splenocyte proliferation, specifically at 200 μg mL(-1). Moreover, in a synergistic stimulation of drugs with LPS or PHA, a significant difference was obtained between the RGPL and RGP at 100-400 μg mL(-1), which indicated that the immunological enhancement of RGP was significantly improved after encapsulation with the liposome.

Keywords: Lymphocyte proliferation; Rehmannia glutinosa polysaccharide liposome; Response surface methodology.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Liposomes / chemistry*
Liposomes / immunology
Lymphocytes / drug effects
Lymphocytes / immunology
Mice
Polysaccharides / chemistry*
Polysaccharides / immunology
Rehmannia / chemistry*

Substances

Liposomes
Polysaccharides