Exploitation of starch industry liquid by-product to produce bioactive peptides from rice hydrolyzed proteins

Lucilla Dei Piu'; Annalisa Tassoni; Diana Isabella Serrazanetti; Maura Ferri; Elena Babini; Davide Tagliazucchi; Andrea Gianotti

doi:10.1016/j.foodchem.2014.01.055

Exploitation of starch industry liquid by-product to produce bioactive peptides from rice hydrolyzed proteins

Food Chem. 2014 Jul 15:155:199-206. doi: 10.1016/j.foodchem.2014.01.055. Epub 2014 Jan 27.

Authors

Lucilla Dei Piu'¹, Annalisa Tassoni², Diana Isabella Serrazanetti³, Maura Ferri², Elena Babini⁴, Davide Tagliazucchi⁵, Andrea Gianotti⁶

Affiliations

¹ Department of Agri-Food Science and Technology (DISTAL), University of Bologna, Via Fanin, 50, 40127 Bologna, Italy.
² Department of Biological, Geological and Environmental Sciences, University of Bologna, Via Irnerio 42, 40126 Bologna, Italy.
³ Inter-departmental Center of Industrial Agri-Food Research (CIRI Agroalimentare), Piazza Goidanich 60, 47521 Cesena, Italy.
⁴ Department of Agri-Food Science and Technology (DISTAL), University of Bologna, Via Fanin, 50, 40127 Bologna, Italy; Inter-departmental Center of Industrial Agri-Food Research (CIRI Agroalimentare), Piazza Goidanich 60, 47521 Cesena, Italy.
⁵ Department of Life Sciences, University of Modena and Reggio Emilia, via Amendola 2, 42122 Reggio Emilia, Italy.
⁶ Department of Agri-Food Science and Technology (DISTAL), University of Bologna, Via Fanin, 50, 40127 Bologna, Italy; Inter-departmental Center of Industrial Agri-Food Research (CIRI Agroalimentare), Piazza Goidanich 60, 47521 Cesena, Italy. Electronic address: andrea.gianotti@unibo.it.

PMID: 24594175
DOI: 10.1016/j.foodchem.2014.01.055

Abstract

Small peptides show higher antioxidant capacity than native proteins and may be absorbed in the intestine without further digestion. In our study, a protein by-product from rice starch industry was hydrolyzed with commercial proteolytic enzymes (Alcalase, Neutrase, Flavourzyme) and microbial whole cells of Bacillus spp. and the released peptides were tested for antioxidant activity. Among enzymes, Alcalase was the most performing, while microbial proteolytic activity was less efficient. Conversely, the antioxidant activity was higher in the samples obtained by microbial hydrolysis and particularly with Bacillus pumilus AG1. The sequences of low molecular weight antioxidant peptides were determined and analyzed for aminoacidic composition. The results obtained so far suggest that the hydrolytic treatment of this industrial by-product, with selected enzymes and microbial systems, can allow its exploitation for the production of functional additives and supplements rich in antioxidant peptides, to be used in new food formulas for human consumption.

Keywords: Antioxidant activity; Bacillus; Peptides; Proteases; Rice endosperm protein.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Antioxidants / chemistry
Bacillus / enzymology
Biocatalysis
Humans
Hydrolysis
Molecular Sequence Data
Oryza / chemistry*
Peptide Hydrolases / chemistry
Peptide Mapping
Peptides / chemistry*
Protein Hydrolysates / chemistry*
Starch / chemistry*
Waste Products / analysis*

Substances

Antioxidants
Peptides
Protein Hydrolysates
Waste Products
Starch
Peptide Hydrolases