The pqqC gene is essential for antifungal activity of Pseudomonas kilonensis JX22 against Fusarium oxysporum f. sp. lycopersici

FEMS Microbiol Lett. 2014 Apr;353(2):98-105. doi: 10.1111/1574-6968.12411. Epub 2014 Apr 11.

Abstract

Strain JX22, exhibiting a broad range of antimicrobial activities to fungal pathogens, was isolated and classified as representing Pseudomonas kilonensis. In this study, the mutant JX22MT1 was obtained by the EZ-Tn5 transposon mutation and showed no antifungal activity against Fusarium oxysporum f. sp. lycopersici as compared with wild-type strain JX22. The pqqC gene was disrupted in the mutant. Antifungal activity at the wild-type level was restored from the mutant JX22MT1 with the introduction of the functional pqqC gene, which encodes pyrroloquinoline-quinone synthesis protein C. The results suggest that pqqC is essential for antifungal activity of P. kilonensis JX22 against F. oxysporum f. sp. lycopersici.

Keywords: antimicrobial activity; bacteria; fungi; mutagenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibiosis
  • Antifungal Agents / isolation & purification*
  • Antifungal Agents / pharmacology
  • Bacterial Proteins / genetics*
  • Bacterial Proteins / isolation & purification
  • Bacterial Proteins / pharmacology
  • Base Sequence
  • Cloning, Molecular
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Fusarium / drug effects*
  • Fusarium / growth & development
  • Molecular Sequence Data
  • Mutagenesis, Insertional
  • Phylogeny
  • Pseudomonas / genetics*
  • Pseudomonas / isolation & purification
  • Pseudomonas / metabolism
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA

Substances

  • Antifungal Agents
  • Bacterial Proteins
  • DNA, Bacterial
  • DNA, Ribosomal
  • PqqC protein, Bacteria
  • RNA, Ribosomal, 16S

Associated data

  • GENBANK/KF840731