Expression profile of fas-fas ligand in spiral ganglion cells during apoptosis

Tae Woo Gu; Woo Yong Bae; Hwan Tae Park; Jae Hoon Lee; Min Young Kang; Sung Wook Jeong; Yoon Kyung Shin

doi:10.3342/ceo.2014.7.1.1

Expression profile of fas-fas ligand in spiral ganglion cells during apoptosis

Clin Exp Otorhinolaryngol. 2014 Mar;7(1):1-6. doi: 10.3342/ceo.2014.7.1.1. Epub 2014 Feb 5.

Authors

Tae Woo Gu¹, Woo Yong Bae¹, Hwan Tae Park², Jae Hoon Lee¹, Min Young Kang¹, Sung Wook Jeong¹, Yoon Kyung Shin²

Affiliations

¹ Department of Otorhinolaryngology-Head and Neck Surgery, Medical Science Research Institute, Dong-A University College of Medicine, Busan, Korea.
² Department of Physiology, Medical Science Research Institute, Dong-A University College of Medicine, Busan, Korea.

Abstract

Objectives: To examine the expression profile of Fas-Fas ligand (FasL) during glutamate (Glu)-induced spiral ganglion cell (SGC) apoptosis.

Methods: Cultured SGCs were treated with 10-mM, 25-mM, and 50-mM concentrations of Glu and incubated for 24 or 48 hours. The expression intensity of FasL, Fas, caspase 3, and morphology of single SGC were evaluated using immunofluorescence staining.

Results: In semiquantitative analysis of the Glu-treated SGC, FasL, and caspase 3 expression intensity were increased with concentration- and time-dependent manner. Fas expression intensity did not change with different concentration at 48 hours. In morphologic analysis of the Glu-treated SGC, number of apoptotic cells were increased with concentration- and time-dependent manner.

Conclusion: FasL was expressed in apoptotic SGCs, suggesting that the Fas-FasL signaling pathway may be involved in the Glu-induced apoptosis of dissociated SGCs.

Keywords: Apoptosis; Caspase 3; Fas ligand; Glutamate; Spiral ganglion cell.