Improved PCR-RFLP method for the identification of Escherichia coli enterohemolysin (ehxA) subtypes

Sandra C Lorenz; Markus Fischer; Julie A Kase

doi:10.1016/j.mimet.2014.02.010

Improved PCR-RFLP method for the identification of Escherichia coli enterohemolysin (ehxA) subtypes

J Microbiol Methods. 2014 May:100:24-6. doi: 10.1016/j.mimet.2014.02.010. Epub 2014 Feb 25.

Authors

Sandra C Lorenz¹, Markus Fischer², Julie A Kase³

Affiliations

¹ U.S. Food and Drug Administration, Center for Food Safety and Applied Nutrition, Division of Microbiology, College Park, MD 20740, USA; University of Hamburg, Hamburg School of Food Science, Institute of Food Chemistry, Grindelallee 117, 20146 Hamburg, Germany. Electronic address: Sandra.Lorenz@fda.hhs.gov.
² University of Hamburg, Hamburg School of Food Science, Institute of Food Chemistry, Grindelallee 117, 20146 Hamburg, Germany.
³ U.S. Food and Drug Administration, Center for Food Safety and Applied Nutrition, Division of Microbiology, College Park, MD 20740, USA.

PMID: 24583193
DOI: 10.1016/j.mimet.2014.02.010

Abstract

Previously published PCR-RFLP methods failed to detect the six genetically different Escherichia coli ehxA subtypes reliably. Here we describe an improved PCR-RFLP method that detects all ehxA subtypes. Importantly, ehxA-subtyping may contribute to the detection of potentially pathogenic STECs and can expedite initial screenings of patient samples and food enrichments.

Keywords: Escherichia coli enterohemolysin; PCR-RFLP; ehxA subtypes.

Published by Elsevier B.V.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Escherichia coli / classification*
Escherichia coli / genetics*
Escherichia coli Proteins / genetics*
Genetic Variation*
Genotype
Genotyping Techniques / methods*
Hemolysin Proteins / genetics*
Polymerase Chain Reaction / methods*
Polymorphism, Restriction Fragment Length*

Substances

Escherichia coli Proteins
Hemolysin Proteins
Hlya protein, E coli