Microfluidic-based manufacture of siRNA-lipid nanoparticles for therapeutic applications

Methods Mol Biol. 2014:1141:109-20. doi: 10.1007/978-1-4939-0363-4_6.

Abstract

A simple, efficient, and scalable manufacturing technique is required for developing siRNA-lipid nanoparticles (siRNA-LNP) for therapeutic applications. In this chapter we describe a novel microfluidic-based manufacturing process for the rapid manufacture of siRNA-LNP, together with protocols for characterizing the size, polydispersity, RNA encapsulation efficiency, RNA concentration, and total lipid concentration of the resultant nanoparticles.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cholesterol / chemistry*
  • Drug Compounding / instrumentation
  • Drug Compounding / methods
  • Drug Delivery Systems / instrumentation
  • Drug Delivery Systems / methods*
  • Humans
  • Microfluidics / instrumentation*
  • Nanoparticles / chemistry*
  • Particle Size
  • Phosphatidylcholines / chemistry*
  • RNA, Small Interfering / chemistry*

Substances

  • Phosphatidylcholines
  • RNA, Small Interfering
  • Cholesterol
  • 1,2-distearoyllecithin