Micropatterning on micropost arrays

Nathan J Sniadecki; Sangyoon J Han; Lucas H Ting; Shirin Feghhi

doi:10.1016/B978-0-12-800281-0.00005-1

Micropatterning on micropost arrays

Methods Cell Biol. 2014:121:61-73. doi: 10.1016/B978-0-12-800281-0.00005-1.

Authors

Nathan J Sniadecki¹, Sangyoon J Han², Lucas H Ting², Shirin Feghhi²

Affiliations

¹ Department of Mechanical Engineering, University of Washington, Seattle, Washington, USA; Department of Bioengineering, University of Washington, Seattle, Washington, USA.
² Department of Mechanical Engineering, University of Washington, Seattle, Washington, USA.

PMID: 24560503
DOI: 10.1016/B978-0-12-800281-0.00005-1

Abstract

Micropatterning of cells can be used in combination with microposts to control cell shape or cell-to-cell interaction while measuring cellular forces. The protocols in this chapter describe how to make SU8 masters for stamps and microposts, how to use soft lithography to replicate these structures in polydimethylsiloxane, and how to functionalize the surface of the microposts for cell attachment.

Keywords: Cell–cell junctions; Mechanobiology; Mechanotransduction; Microcontact printing; Micropillars; Microposts; Nanoposts; Traction forces; Tugging forces.

MeSH terms

Animals
Cell Adhesion / physiology
Cell Communication
Cell Culture Techniques
Cells, Cultured
Coated Materials, Biocompatible*
Epoxy Compounds / chemistry
Hydrophobic and Hydrophilic Interactions
Mechanotransduction, Cellular / physiology*
Microtechnology / methods*
Polymers / chemistry
Stress, Mechanical
Surface Properties

Substances

Coated Materials, Biocompatible
Epoxy Compounds
Polymers
SU-8 compound