Neolignans as xanthine oxidase inhibitors from Hyptis rhomboides

Phytochemistry. 2014 May:101:121-7. doi: 10.1016/j.phytochem.2014.01.016. Epub 2014 Feb 19.

Abstract

The active fraction from the EtOH extract of Hyptis rhomboides against xanthine oxidase was identified by use of an HPLC microfractionation-centrifugal vacuum evaporation-bioassay hyphenated technique. Scale-up separation of the active subfractions using semi-preparative RP-HPLC provided 13 phenylpropanoid compounds, including O-styrenylneolignans, hyprhombins A-C, epihyprhombin B, and hyprhombin B methyl ester, and O-caffeoylnorneolignans, hyprhombins D and E. All of these compounds shared a common 1,4-benzodioxane skeleton, as established by spectroscopic analyses. Hyprhombin C and epihyprhombin B exhibited better anti-xanthine oxidase activity than allopurinol, with IC50 values of 0.6 and 2.0 μM, respectively.

Keywords: 1,4-Benzodioxane; HPLC microfractionation–centrifugal vacuum evaporation–bioassay hyphenation; Hyprhombins; Hyptis rhomboides; Labiatae; Neolignans; Xanthine oxidase inhibitor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, High Pressure Liquid
  • Drugs, Chinese Herbal / isolation & purification
  • Drugs, Chinese Herbal / pharmacology*
  • Enzyme Inhibitors / isolation & purification
  • Enzyme Inhibitors / pharmacology*
  • Hyptis / chemistry*
  • Inhibitory Concentration 50
  • Lignans / isolation & purification
  • Lignans / pharmacology*
  • Molecular Structure
  • Plant Components, Aerial / chemistry
  • Xanthine Oxidase / antagonists & inhibitors*

Substances

  • Drugs, Chinese Herbal
  • Enzyme Inhibitors
  • Lignans
  • Xanthine Oxidase