Abstract
INI1/hSNF5/BAF47, which has an SNF5 domain, belongs to the SWI/SNF family. This family is known as ATP-dependent regulators of gene expression by remodeling chromatin structure during cell differentiation. However, the detailed function of INI1/hSNF5/BAF47 is unclear. Here we report the generation of a specific monoclonal antibody for INI1/hSNF5/BAF47 by the mouse iliac lymph node method. The obtained antibody recognized two isoforms of INI1/hSNF5/BAF47 in immunoblotting and precisely recognized the nuclear localization of INI1/hSNF5/BAF47 in immunostaining. This antibody can contribute to further elucidation of the mechanisms of gene expression regulation by INI1/hSNF5/BAF47 during cell differentiation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Monoclonal / biosynthesis*
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Antibodies, Monoclonal / immunology*
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Cell Differentiation / genetics
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Cell Differentiation / immunology
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Cell Line, Tumor
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Chromosomal Proteins, Non-Histone / immunology*
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DNA-Binding Proteins / immunology*
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Female
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Gene Expression Regulation / genetics
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Gene Expression Regulation / immunology
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Glutathione Transferase / genetics
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HeLa Cells
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Humans
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Hybridomas / cytology
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Hybridomas / immunology
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Lymph Nodes / cytology
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Lymph Nodes / immunology
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Mice
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Mice, Inbred ICR
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Protein Isoforms / genetics
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Protein Isoforms / immunology
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Recombinant Fusion Proteins / genetics
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SMARCB1 Protein
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Transcription Factors / immunology*
Substances
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Antibodies, Monoclonal
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Chromosomal Proteins, Non-Histone
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DNA-Binding Proteins
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Protein Isoforms
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Recombinant Fusion Proteins
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SMARCB1 Protein
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SMARCB1 protein, human
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Transcription Factors
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Glutathione Transferase