Mercury arc lamp based super-resolution imaging with conventional fluorescence microscopes

Zhiwei Yuan; Jielin Sun; Renkuan Zhai; Xiaowei Li; Zhifeng Shao

doi:10.1016/j.micron.2013.11.006

Mercury arc lamp based super-resolution imaging with conventional fluorescence microscopes

Micron. 2014 Apr:59:24-7. doi: 10.1016/j.micron.2013.11.006. Epub 2013 Dec 7.

Authors

Zhiwei Yuan¹, Jielin Sun¹, Renkuan Zhai², Xiaowei Li³, Zhifeng Shao⁴

Affiliations

¹ Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200240, China.
² School of Biomedical Engineering and Bio-ID Center, Shanghai Jiao Tong University, Shanghai 200240, China.
³ School of Biomedical Engineering and Bio-ID Center, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: xl3a@sjtu.edu.cn.
⁴ Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200240, China; School of Biomedical Engineering and Bio-ID Center, Shanghai Jiao Tong University, Shanghai 200240, China; State Key Laboratory for Oncogene and Related Genes, Shanghai Jiao Tong University, Shanghai 200240, China.

PMID: 24530361
DOI: 10.1016/j.micron.2013.11.006

Abstract

We present an implementation of localization based three-dimensional super-resolution imaging on a regular microscope. We retain the original arc lamp as the photoactivation light source, and incorporate an inexpensive diode laser for imaging. As alterations to the standard microscope is minimal, this optical setup can be easily adapted in a typical research laboratory and even undergraduate teaching experiments, providing an inexpensive system for students and research scientists who require such super resolution capabilities. With this simple design, a spatial resolution of better than 40 nm at a reasonable frame rate has been achieved, adequate for most routine applications.

Keywords: Localization-based super-resolution microscopy; Mercury arc lamp; Photoactivation source.

Publication types

Research Support, Non-U.S. Gov't