Analysis of adipose tissue lipid using mass spectrometry

Rodney C Baker; Yana Nikitina; Angela R Subauste

doi:10.1016/B978-0-12-800280-3.00006-2

Analysis of adipose tissue lipid using mass spectrometry

Methods Enzymol. 2014:538:89-105. doi: 10.1016/B978-0-12-800280-3.00006-2.

Authors

Rodney C Baker¹, Yana Nikitina², Angela R Subauste³

Affiliations

¹ Department of Pharmacology and Toxicology, University of Mississippi Medical Center, Jackson, Mississippi, USA.
² Division of Endocrinology, Department of Medicine, University of Mississippi Medical Center, Jackson, Mississippi, USA.
³ Division of Endocrinology, Department of Medicine, University of Mississippi Medical Center, Jackson, Mississippi, USA. Electronic address: asubauste@umc.edu.

PMID: 24529435
DOI: 10.1016/B978-0-12-800280-3.00006-2

Abstract

Mass spectrometry technology has enabled significant advances in detailing the alterations of the lipidome in response to pathological conditions or experimental manipulations. Lipids comprise a wide range of compounds with functions that include structural, intracellular signaling, trafficking, and storage. Characterization of lipid species has evolved significantly over recent years due to the progress made in the area of mass spectrometry. This chapter details the methods used for the analysis of lipids tailored to the intrinsic characteristics of adipose tissue. Particular attention is given to the analysis of triglycerides, diacylglycerols, and phospholipid.

Keywords: Cyclic phosphatidic acid; Diacylglycerides; Lysophosphatidic acid; Phospholipids; Triglycerides.

MeSH terms

Adipose Tissue / chemistry*
Chromatography, Liquid / methods
Diglycerides / analysis
Humans
Lipids / analysis*
Mass Spectrometry / methods*
Phospholipids / analysis
Triglycerides / analysis

Substances

Diglycerides
Lipids
Phospholipids
Triglycerides

Grants and funding

R03 DK092542/DK/NIDDK NIH HHS/United States