The effects of titania nanotubes with embedded silver oxide nanoparticles on bacteria and osteoblasts

Ang Gao; Ruiqiang Hang; Xiaobo Huang; Lingzhou Zhao; Xiangyu Zhang; Lin Wang; Bin Tang; Shengli Ma; Paul K Chu

doi:10.1016/j.biomaterials.2014.01.058

The effects of titania nanotubes with embedded silver oxide nanoparticles on bacteria and osteoblasts

Biomaterials. 2014 Apr;35(13):4223-35. doi: 10.1016/j.biomaterials.2014.01.058. Epub 2014 Feb 13.

Authors

Ang Gao¹, Ruiqiang Hang², Xiaobo Huang¹, Lingzhou Zhao³, Xiangyu Zhang¹, Lin Wang⁴, Bin Tang¹, Shengli Ma⁵, Paul K Chu⁶

Affiliations

¹ Research Institute of Surface Engineering, Taiyuan University of Technology, Taiyuan 030024, China.
² Research Institute of Surface Engineering, Taiyuan University of Technology, Taiyuan 030024, China. Electronic address: hangruiqiang@tyut.edu.cn.
³ Department of Periodontology & Oral Medicine, School of Stomatology, The Fourth Military Medical University, Xi'an 710032, China. Electronic address: zhaolingzhou1983@hotmail.com.
⁴ Institute of Materials, Ningbo University of Technology, Ningbo 315016, China.
⁵ State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an 710049, China.
⁶ Department of Physics and Materials Science, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, China.

PMID: 24529392
DOI: 10.1016/j.biomaterials.2014.01.058

Abstract

A versatile strategy to endow biomaterials with long-term antibacterial ability without compromising the cytocompatibility is highly desirable to combat biomaterial related infection. TiO2 nanotube (NT) arrays can significantly enhance the functions of many cell types including osteoblasts thus having promising applications in orthopedics, orthodontics, as well as other biomedical fields. In this study, TiO2 NT arrays with Ag2O nanoparticle embedded in the nanotube wall (NT-Ag2O arrays) are prepared on titanium (Ti) by TiAg magnetron sputtering and anodization. Well-defined NT arrays containing Ag concentrations in a wide range from 0 to 15 at % are formed. Ag incorporation has little influence on the NT diameter, but significantly decreases the tube length. Crystallized Ag2O nanoparticles with diameters ranging from 5 nm to 20 nm are embedded in the amorphous TiO2 nanotube wall and this unique structure leads to controlled release of Ag(+) that generates adequate antibacterial activity without showing cytotoxicity. The NT-Ag2O arrays can effectively kill Escherichia coli and Staphylococcus aureus even after immersion for 28 days, demonstrating the long lasting antibacterial ability. Furthermore, the NT-Ag2O arrays have no appreciable influence on the osteoblast viability, proliferation, and differentiation compared to the Ag free TiO2 NT arrays. Ag incorporation even shows some favorable effects on promoting cell spreading. The technique reported here is a versatile approach to develop biomedical coatings with different functions.

Keywords: Ag(2)O nanoparticles; Anodization; Antibacterial activity; Osteoblasts; TiO(2) nanotubes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / chemistry*
Anti-Bacterial Agents / pharmacology*
Escherichia / drug effects
Nanotubes / chemistry*
Osteoblasts / drug effects*
Oxides / chemistry*
Silver Compounds / chemistry*
Staphylococcus aureus / drug effects
Titanium / chemistry*

Substances

Anti-Bacterial Agents
Oxides
Silver Compounds
titanium dioxide
disilver oxide
Titanium