The polysaccharides from fermented Ganoderma lucidum mycelia induced miRNAs regulation in suppressed HepG2 cells

Carbohydr Polym. 2014 Mar 15:103:319-24. doi: 10.1016/j.carbpol.2013.12.044. Epub 2013 Dec 21.

Abstract

Medicinal mushroom polysaccharides such as Ganoderma lucidum polysaccharides (GLPs) have been commonly hypothesized to suppress tumor cells proliferation through immune effects. To verify this hypothesis through investigating comprehensive miRNA expression in polysaccharide treated cancer cells, an anticancer mycelia GLP was employed to disclose miRNA differential expression of human hepatocarcinoma cells (HepG2), by using a miRNA microarray assay based on Sanger miR-Base Release 16. The experiment and the analysis result indicates that among the 61 differential expressed miRNAs (p ≤ 0.01), 17 of them were regulated significantly. GLP can inhibit HepG2 cells directly through regulation of hepatocarcinoma genes. A newly found miR-3131 exhibited the strongest upregulation (92-folds, Log2 = 6.53, p = 0.000016). The miRNAs responded synergistically in both hepatocarcinoma and immune-related aspects.

Keywords: Antitumor; Ganoderma lucidum; Hepatocarcinoma; Immune; Polysaccharide; miRNA.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Fermentation*
  • Hep G2 Cells
  • Humans
  • MicroRNAs / genetics
  • MicroRNAs / metabolism*
  • Polysaccharides / chemistry
  • Polysaccharides / metabolism*
  • Reishi / chemistry
  • Reishi / metabolism*

Substances

  • MicroRNAs
  • Polysaccharides