Polish population data on 15 autosomal STRs of AmpFlSTR NGM PCR kit

Ireneusz Soltyszewski; Witold Pepinski; Paulina Wolanska-Nowak; Agnieszka Maciejewska; Regina Paszkowska; Monica Abreu-Glowacka; Wojciech Achrem; Anna Jonkisz; Arleta Lebioda; Magdalena Konarzewska; Rafal Ploski

doi:10.1016/j.fsigen.2013.12.002

Polish population data on 15 autosomal STRs of AmpFlSTR NGM PCR kit

Forensic Sci Int Genet. 2014 Mar:9:142-9. doi: 10.1016/j.fsigen.2013.12.002. Epub 2013 Dec 13.

Affiliations

¹ Department of Criminalistics and Forensic Medicine, University of Warmia and Mazury, Olsztyn, Poland. Electronic address: ireneusz.soltyszewski@uwm.edu.pl.
² Department of Forensic Medicine, Medical University of Bialystok, Poland.
³ Forensic Genetics Section, Institute of Forensic Research in Krakow, Poland.
⁴ Department of Forensic Medicine, Medical University of Gdansk, Poland.
⁵ Department of Forensic Medicine, Medical University of Poznan, Poland.
⁶ Regional Forensic Laboratory of Police, Szczecin, Poland.
⁷ Institute of Molecular Techniques, Medical University of Wroclaw, Poland.
⁸ Department of Forensic Medicine, Warsaw Medical University, Poland.
⁹ Department of Medical Genetics, Warsaw Medical University, Poland.

PMID: 24528592
DOI: 10.1016/j.fsigen.2013.12.002

Abstract

The objective of the research was to provide a comprehensive database of autosomal microsatellite loci included in AmpFlSTR NGM PCR kit for a population of Poland considering possible genetic differentiation of a forensic interest. Fifteen STR markers were analyzed in 2041 unrelated individuals residing in eight geographically different regions. All the loci were found to be in Hardy-Weinberg equilibrium. The combined probability of match is 3.52 × 10(-19) and the combined Power of Exclusion is 0.9999998. The F(ST) estimate over all 15 STRs is 0.0051 for the Polish population. We established that a combined NGM database may be employed for a Polish population.

Keywords: AmpFlSTR NGM; Forensic genetics; Poland; Population; STR.

MeSH terms

DNA Fingerprinting*
Gene Frequency
Genetics, Population*
Humans
Microsatellite Repeats*
Poland
Real-Time Polymerase Chain Reaction