A case study of SNPSTR efficiency in paternity testing with locus incompatibility

Yi Ye; Haibo Luo; Linchuan Liao; Ji Zhang; Wei Wei; Zheng Wang; Yiping Hou

doi:10.1016/j.fsigen.2013.11.004

A case study of SNPSTR efficiency in paternity testing with locus incompatibility

Forensic Sci Int Genet. 2014 Mar:9:72-5. doi: 10.1016/j.fsigen.2013.11.004. Epub 2013 Dec 4.

Authors

Yi Ye¹, Haibo Luo², Linchuan Liao³, Ji Zhang², Wei Wei², Zheng Wang², Yiping Hou⁴

Affiliations

¹ Department of Forensic Biology, West China School of Basic Science and Forensic Medicine, Sichuan University, Chengdu 610041, Sichuan, China; Department of Forensic Analytical Toxicology, West China School of Basic Science and Forensic Medicine, Sichuan University, Chengdu 610041, Sichuan, China.
² Department of Forensic Biology, West China School of Basic Science and Forensic Medicine, Sichuan University, Chengdu 610041, Sichuan, China.
³ Department of Forensic Analytical Toxicology, West China School of Basic Science and Forensic Medicine, Sichuan University, Chengdu 610041, Sichuan, China.
⁴ Department of Forensic Biology, West China School of Basic Science and Forensic Medicine, Sichuan University, Chengdu 610041, Sichuan, China. Electronic address: prof.hou@gmail.com.

PMID: 24528583
DOI: 10.1016/j.fsigen.2013.11.004

Abstract

Instead of testing the additional STR loci, SNPSTR was included in the paternity testing for an alleged father-son duo case, with one inconsistency at the CSF1PO locus. We have chosen CSF1PO STR and five closely linked SNPs rs10077461, rs2569076, rs2228422, rs3733673 and rs3829987 to establish the SNPSTR and examined its potential use in paternity testing. A total of 152 haplotypes from 76 unrelated individuals were obtained by the nested-AS-PCR and 60 SNPSTR haplotypes were observed. The discrimination power of the SNPSTR haplotype was greater than either the STR or SNP haplotype alone. Its SNP part could be used to distinguish fathers from uncles. When SNPSTR was introduced into the calculation of parentage statistics, the paternity probability increased to 99.998%. Based on our findings, we concluded that SNPSTR could be considered a useful tool in forensic science.

Keywords: CSF1PO; Mutation; Paternity testing; SNPSTR.

Publication types

Case Reports
Research Support, Non-U.S. Gov't

MeSH terms

Haplotypes
Humans
Male
Microsatellite Repeats*
Multiplex Polymerase Chain Reaction
Paternity*
Polymorphism, Single Nucleotide*
Sequence Analysis, DNA