A sensitive colorimetric high-throughput screening method for lipase synthetic activity assay

Jianyong Zheng; Xianfeng Fu; Xiangxian Ying; Yinjun Zhang; Zhao Wang

doi:10.1016/j.ab.2014.02.001

A sensitive colorimetric high-throughput screening method for lipase synthetic activity assay

Anal Biochem. 2014 May 1:452:13-5. doi: 10.1016/j.ab.2014.02.001. Epub 2014 Feb 10.

Authors

Jianyong Zheng¹, Xianfeng Fu¹, Xiangxian Ying¹, Yinjun Zhang¹, Zhao Wang²

Affiliations

¹ College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, People's Republic of China.
² College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, People's Republic of China. Electronic address: hzwangzhao@163.com.

PMID: 24525041
DOI: 10.1016/j.ab.2014.02.001

Abstract

A sensitive and practical high-throughput screening method for assaying lipase synthetic activity is described. Lipase-catalyzed transesterification between vinyl acetate and n-butanol in n-hexane was chosen as a model reaction. The released acetaldehyde was determined by the colorimetric method using 3-methyl-2-benzothialinone (MBTH) derivatization. In comparison with other methods, the major advantages of this process include high sensitivity, simple detection, inexpensive reagents, and low requirements for instruments.

Keywords: Derivatization; High-throughput screening; Lipase; MBTH; Synthetic activity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Butanols / metabolism
Colorimetry / methods*
Enzyme Assays / methods*
High-Throughput Screening Assays
Lipase / metabolism*
Vinyl Compounds / metabolism

Substances

Butanols
Vinyl Compounds
Lipase
vinyl acetate