The sulfated O-specific polysaccharide from the marine bacterium Cobetia pacifica KMM 3879(T.)

Maxim S Kokoulin; Anatoliy I Kalinovsky; Nadezhda A Komandrova; Svetlana V Tomshich; Lyudmila A Romanenko; Victor E Vaskovsky

doi:10.1016/j.carres.2014.01.015

The sulfated O-specific polysaccharide from the marine bacterium Cobetia pacifica KMM 3879(T.)

Carbohydr Res. 2014 Mar 31:387:4-9. doi: 10.1016/j.carres.2014.01.015. Epub 2014 Jan 27.

Authors

Maxim S Kokoulin¹, Anatoliy I Kalinovsky², Nadezhda A Komandrova², Svetlana V Tomshich², Lyudmila A Romanenko², Victor E Vaskovsky³

Affiliations

¹ G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far East Branch of the Russian Academy of Sciences, Vladivostok, Russia. Electronic address: maxchem@mail.ru.
² G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far East Branch of the Russian Academy of Sciences, Vladivostok, Russia.
³ G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far East Branch of the Russian Academy of Sciences, Vladivostok, Russia; Far Eastern Federal University, Vladivostok, Russia.

PMID: 24518985
DOI: 10.1016/j.carres.2014.01.015

Abstract

The O-specific polysaccharide was isolated from the lipopolysaccharide of Cobetia pacifica KMM 3879(T) and studied by chemical methods along with (1)H and (13)C NMR spectroscopy, including 1D TOCSY and 2D (1)H, (1)H-COSY, ROESY, (1)H, (13)C-HSQC, HMBC, H2BC and HMQC-TOCSY experiments. The following new structure of the sulfated O-polysaccharide from the C. pacifica KMM 3879(T) containing rhamnose (Rha), glucose (Glc), and galactose (Gal) was established: where R is -SO3H.

Keywords: Cobetia pacifica; Marine bacterium; O-Specific polysaccharide; Sulfate.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Galactose / chemistry
Glucose / chemistry
Halomonadaceae / chemistry*
Magnetic Resonance Spectroscopy
O Antigens / chemistry*
O Antigens / isolation & purification
Rhamnose / chemistry
Sulfates / chemistry*

Substances

O Antigens
Sulfates
Glucose
Rhamnose
Galactose