Polyvalent display of monosaccharides on ferritin protein cage nanoparticles for the recognition and binding of cell-surface lectins

Abstract

Carbohydrate-lectin interactions are important in many biological events. Endogenous cell-surface lectins are attractive markers for the recognition and targeting. Human ferritin protein cage nanoparticles (HFPCNs) are prepared as delivery nanoplatforms and two different types of monosaccharide derivatives; maleimido group terminated-mannopyranoside and galactopyranoside. Uniform and polyvalent displays of mannoses or galactoses on the surface of HFPCNs are achieved by using site-specific thiol-maleimide Michael-type addition. Mannose- or galactose-displaying HFPCNs recognize and tightly bind to DC-SIGN or ASGP-R lectins on the surface of the mammalian cells, DCEK or HepG2 cells.

Keywords: cell-surface lectins; delivery nanoplatforms; glycoconjugates; polyvalent display; protein cages.