Generation and use of antibody fragments for structural studies of proteins refractory to crystallization

Stephen J Stahl; Norman R Watts; Paul T Wingfield

doi:10.1007/978-1-62703-992-5_35

Generation and use of antibody fragments for structural studies of proteins refractory to crystallization

Methods Mol Biol. 2014:1131:549-61. doi: 10.1007/978-1-62703-992-5_35.

Authors

Stephen J Stahl¹, Norman R Watts, Paul T Wingfield

Affiliation

¹ Protein Expression Laboratory, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD, USA.

Abstract

With the rapid technological advances in all aspects of macromolecular X-ray crystallography the preparation of diffraction quality crystals has become the rate-limiting step. Crystallization chaperones have proven effective for overcoming this barrier. Here we describe the usage of a Fab chaperone for the crystallization of HIV-1 Rev, a protein that has long resisted all attempts at elucidating its complete atomic structure.

Publication types

Research Support, N.I.H., Intramural

MeSH terms

Biotechnology / methods*
Crystallography, X-Ray*
Gene Products, rev / metabolism*
HIV-1 / metabolism*

Substances

Gene Products, rev

Grants and funding

ZIA AR041115-18/Intramural NIH HHS/United States