Abstract
miR-625 has been reported to exhibit abnormal expression in esophageal cancer (EC), but the mechanism and functions of miR-625 in esophageal cancer remain unclear. miR-625 down-regulation and Sox2 up-regulation were validated by qRT-PCR in 158 EC samples. Low expression of miR-625 promotes cell proliferation and invasion, while high expression of miR-625 has the opposite effect. Sox2, a target gene of miR-625, was examined by luciferase assay and western blot. Our data suggest that miR-625 may regulate the biological processes of EC via controlling Sox2 expression.
Keywords:
Esophageal cancer; Invasion; Proliferation; Sox2; miR-625.
Copyright © 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
MeSH terms
-
3' Untranslated Regions
-
Adenocarcinoma / genetics
-
Adenocarcinoma / metabolism*
-
Adenocarcinoma / secondary
-
Aged
-
Apoptosis
-
Base Sequence
-
Binding Sites
-
Carcinoma, Squamous Cell / genetics
-
Carcinoma, Squamous Cell / metabolism*
-
Carcinoma, Squamous Cell / secondary
-
Cell Line, Tumor
-
Cell Movement
-
Cell Proliferation
-
Down-Regulation
-
Esophageal Neoplasms / genetics
-
Esophageal Neoplasms / metabolism*
-
Esophageal Neoplasms / pathology
-
Female
-
Gene Expression Regulation, Neoplastic*
-
Humans
-
Male
-
MicroRNAs / genetics*
-
MicroRNAs / metabolism
-
Middle Aged
-
Neoplasm Invasiveness
-
RNA Interference
-
SOXB1 Transcription Factors / genetics*
-
SOXB1 Transcription Factors / metabolism
Substances
-
3' Untranslated Regions
-
MIRN625 microRNA, human
-
MicroRNAs
-
SOX2 protein, human
-
SOXB1 Transcription Factors