Detection of Anaplasma platys in dogs using real-time loop-mediated isothermal amplification

Vet J. 2014 Mar;199(3):468-70. doi: 10.1016/j.tvjl.2013.11.012. Epub 2013 Nov 22.

Abstract

Anaplasma platys is a parasite of canine platelets that causes infectious cyclic thrombocytopenia. In this study, a novel real-time loop-mediated isothermal amplification (RT-LAMP) method was developed to detect A. platys. RT-LAMP primer sets were designed using a citrate synthase gene sequence and the assay was performed at 63 °C for 30 min. No cross-reactivity was observed with other Anaplasma or Ehrlichia spp. and the method exhibited a similar level of sensitivity in detecting the organism in 58 canine blood samples to that of a nested PCR. This RT-LAMP is a rapid and potentially cost-effective method of diagnosing A. platys infection in dogs.

Keywords: Anaplasma platys; Canine; Real-time loop-mediated isothermal amplification; gltA gene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anaplasma / genetics
  • Anaplasma / isolation & purification*
  • Anaplasmosis / diagnosis*
  • Anaplasmosis / parasitology
  • Animals
  • China
  • Dog Diseases / diagnosis*
  • Dog Diseases / parasitology
  • Dogs
  • Ehrlichia / genetics
  • Ehrlichia / isolation & purification
  • Nucleic Acid Amplification Techniques / economics
  • Nucleic Acid Amplification Techniques / veterinary*
  • Sensitivity and Specificity