Cycloamylose production from amylomaize by isoamylase and Thermus aquaticus 4-α-glucanotransferase

Yan Xu; Xing Zhou; Yuxiang Bai; Jinpeng Wang; Chunsen Wu; Xueming Xu; Zhengyu Jin

doi:10.1016/j.carbpol.2013.10.065

Cycloamylose production from amylomaize by isoamylase and Thermus aquaticus 4-α-glucanotransferase

Carbohydr Polym. 2014 Feb 15:102:66-73. doi: 10.1016/j.carbpol.2013.10.065. Epub 2013 Nov 22.

Authors

Yan Xu¹, Xing Zhou², Yuxiang Bai³, Jinpeng Wang⁴, Chunsen Wu⁵, Xueming Xu⁶, Zhengyu Jin⁷

Affiliations

¹ State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi 214122, China. Electronic address: xuyan19890225@163.com.
² State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi 214122, China. Electronic address: zhouxing@jiangnan.edu.cn.
³ Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Nijenborgh 7, 9747 AG Groningen, The Netherlands. Electronic address: y.bai@rug.nl.
⁴ State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China. Electronic address: wangjinpeng@jiangnan.edu.cn.
⁵ State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China. Electronic address: wuchunsen@126.com.
⁶ State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi 214122, China. Electronic address: xmxu@jiangnan.edu.cn.
⁷ State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi 214122, China. Electronic address: jinlab2008@yahoo.com.

PMID: 24507256
DOI: 10.1016/j.carbpol.2013.10.065

Abstract

Amylomaize (AMM) was utilized as the substrate for cycloamylose (CA) production in this study. After debranching, AMM was incubated with 10 U/g of Thermus aquaticus 4-α-glucanotransferase (TA 4αGTase) for various reaction times in water or in DMSO reaction system. The maximum conversion yield of CA was greatly improved from 24.55% to 45.58% and from 27.40% to 47.25% after debranching in the water and DMSO reaction systems, respectively. Compared with the method that produced CA from commercial potato amylose, this method produced CA from a natural amylopectin containing starch with enhanced conversion yield after debranching. Meanwhile, we found that the minimum degree of polymerization (DP) of CA from TA 4αGTase treatment was 5, regardless of the reaction conditions. These results were different from those reported in the literature that stated the minimum DP of CA produced with a TA 4αGTase treatment was 22 regardless of the reaction conditions.

Keywords: Amylomaize; Cycloamylose; Isoamylase; Thermus aquaticus 4-α-glucanotransferase.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cyclodextrins / biosynthesis*
Dimethyl Sulfoxide / chemistry
Glycogen Debranching Enzyme System / metabolism*
Isoamylase / metabolism*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Starch / metabolism*
Thermus / enzymology*

Substances

Cyclodextrins
Glycogen Debranching Enzyme System
Starch
4 alpha-glucanotransferase
Isoamylase
Dimethyl Sulfoxide