Effects of selenylation modification on immune-enhancing activity of garlic polysaccharide

Shulei Qiu; Jin Chen; Tao Qin; Yuanliang Hu; Deyun Wang; Qiang Fan; Cunshuai Zhang; Xingying Chen; Xiaolan Chen; Cui Liu; Zhenzhen Gao; Xiuping Li

doi:10.1371/journal.pone.0086377

Effects of selenylation modification on immune-enhancing activity of garlic polysaccharide

PLoS One. 2014 Jan 30;9(1):e86377. doi: 10.1371/journal.pone.0086377. eCollection 2014.

Authors

Shulei Qiu¹, Jin Chen², Tao Qin¹, Yuanliang Hu¹, Deyun Wang¹, Qiang Fan³, Cunshuai Zhang³, Xingying Chen¹, Xiaolan Chen¹, Cui Liu¹, Zhenzhen Gao¹, Xiuping Li¹

Affiliations

¹ Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
² National Research Center of Veterinary Biological Engineering and Technology, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
³ China Institute of Veterinary Drug Control, Beijing, China.

Abstract

The garlic polysaccharide was modified by HNO3-Na2SeO3 method according to orthogonal design L9(3(4)) to obtain nine selenizing garlic polysaccharides, sGPS1-sGPS9. Their effects on chicken peripheral lymphocytes proliferation in vitro were compared by MTT assay. The results showed that sGPSs could significantly promote lymphocytes proliferation, sGPS3, sGPS5 and sGPS6 presented stronger efficacy. In vivo experiment, 14-day-old chickens were injected respectively with sGPS3, sGPS5 and sGPS6 when they were vaccinated with ND vaccine taking unmodified GPS as control. The results showed that three sGPSs could significantly promote lymphocyte proliferation, enhance serum antibody titer, IFN-γ and IL-2 contents. These results indicated that selenylation modification could significantly enhance the immune-enhancing activity of GPS, sGPS6 possessed the best efficacy and could be as a candidate drug of immunoenhancer. Its optimal modification conditions were 400 mg of sodium selenite for 500 mg of GPS, reaction temperature of 70°C and reaction time of 6 h.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adjuvants, Immunologic / chemistry
Adjuvants, Immunologic / pharmacology*
Animals
Antibodies, Viral / blood
Avian Proteins / blood
Cell Proliferation
Cells, Cultured
Chickens
Garlic / chemistry*
Interferon-gamma / blood
Interleukin-2 / blood
Lymphocytes / immunology
Lymphocytes / physiology
Male
Newcastle disease virus / immunology
Organoselenium Compounds / chemistry
Organoselenium Compounds / pharmacology*
Plant Extracts / chemistry
Plant Extracts / pharmacology*
Polysaccharides / chemistry
Polysaccharides / pharmacology*
Viral Vaccines / immunology

Substances

Adjuvants, Immunologic
Antibodies, Viral
Avian Proteins
Interleukin-2
Organoselenium Compounds
Plant Extracts
Polysaccharides
Viral Vaccines
Interferon-gamma

Grants and funding

The project was supported by National Natural Science Foundation of China (31272596), National Technological Supporting Program of China (2011BAD34B02), and a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.