Biodegradable polymeric vesicles containing magnetic nanoparticles, quantum dots and anticancer drugs for drug delivery and imaging

Fei Ye; Asa Barrefelt; Heba Asem; Manuchehr Abedi-Valugerdi; Ibrahim El-Serafi; Maryam Saghafian; Khalid Abu-Salah; Salman Alrokayan; Mamoun Muhammed; Moustapha Hassan

doi:10.1016/j.biomaterials.2014.01.041

Biodegradable polymeric vesicles containing magnetic nanoparticles, quantum dots and anticancer drugs for drug delivery and imaging

Biomaterials. 2014 Apr;35(12):3885-94. doi: 10.1016/j.biomaterials.2014.01.041. Epub 2014 Feb 1.

Affiliations

¹ Division of Experimental Cancer Medicine, Department of Laboratory Medicine (LABMED), Karolinska Institutet, SE-141 86 Stockholm, Sweden.
² King Abdullah Institute for Nanotechnology, King Saud University, Riyadh 11451, P.O. Box 2455, Saudi Arabia. Electronic address: abusalah@ksu.edu.sa.
³ King Abdullah Institute for Nanotechnology, King Saud University, Riyadh 11451, P.O. Box 2455, Saudi Arabia.
⁴ Division of Functional Materials, School of Information and Communication Technology, Royal Institute of Technology (KTH), SE-164 40 Stockholm, Sweden.
⁵ Division of Experimental Cancer Medicine, Department of Laboratory Medicine (LABMED), Karolinska Institutet, SE-141 86 Stockholm, Sweden; Clinical Research Center, Karolinska University Hospital - Huddinge, SE-141 86 Stockholm, Sweden. Electronic address: moustapha.hassan@ki.se.

PMID: 24495486
DOI: 10.1016/j.biomaterials.2014.01.041

Abstract

We have developed biodegradable polymeric vesicles as a nanocarrier system for multimodal bio-imaging and anticancer drug delivery. The poly(lactic-co-glycolic acid) (PLGA) vesicles were fabricated by encapsulating inorganic imaging agents of superparamagnetic iron oxide nanoparticles (SPION), manganese-doped zinc sulfide (Mn:ZnS) quantum dots (QDs) and the anticancer drug busulfan into PLGA nanoparticles via an emulsion-evaporation method. T2∗-weighted magnetic resonance imaging (MRI) of PLGA-SPION-Mn:ZnS phantoms exhibited enhanced negative contrast with r2∗ relaxivity of approximately 523 s(-1) mM(-1) Fe. Murine macrophage (J774A) cellular uptake of PLGA vesicles started fluorescence imaging at 2 h and reached maximum intensity at 24 h incubation. The drug delivery ability of PLGA vesicles was demonstrated in vitro by release of busulfan. PLGA vesicle degradation was studied in vitro, showing that approximately 32% was degraded into lactic and glycolic acid over a period of 5 weeks. The biodistribution of PLGA vesicles was investigated in vivo by MRI in a rat model. Change of contrast in the liver could be visualized by MRI after 7 min and maximal signal loss detected after 4 h post-injection of PLGA vesicles. Histological studies showed that the presence of PLGA vesicles in organs was shifted from the lungs to the liver and spleen over time.

Keywords: Anticancer drug delivery; Biodegradable polymer; Busulfan; Fluorescence imaging; Magnetic resonance imaging; Multifunctional nanoparticles.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antineoplastic Agents / administration & dosage*
Biocompatible Materials*
Drug Carriers*
Lactic Acid / chemistry
Magnetic Resonance Imaging
Magnetics*
Metal Nanoparticles
Mice
Polyglycolic Acid / chemistry
Polylactic Acid-Polyglycolic Acid Copolymer
Polymers / chemistry*
Quantum Dots*
Rats
Tissue Distribution

Substances

Antineoplastic Agents
Biocompatible Materials
Drug Carriers
Polymers
Polylactic Acid-Polyglycolic Acid Copolymer
Polyglycolic Acid
Lactic Acid