Using high-performance liquid chromatography with UV detector to quantify exhaled leukotriene B4 level in nonatopic adults

Gwo-Hwa Wan; Dah-Chin Yan; Hui-Yun Tseng; Jian-Tao Lee; Yu-Wen Lin

doi:10.1016/j.jfma.2013.12.009

Using high-performance liquid chromatography with UV detector to quantify exhaled leukotriene B4 level in nonatopic adults

J Formos Med Assoc. 2014 Aug;113(8):566-8. doi: 10.1016/j.jfma.2013.12.009. Epub 2014 Feb 1.

Authors

Gwo-Hwa Wan¹, Dah-Chin Yan², Hui-Yun Tseng³, Jian-Tao Lee⁴, Yu-Wen Lin⁵

Affiliations

¹ Department of Respiratory Therapy, College of Medicine, Chang Gung University, Taoyuan, Taiwan.
² Division of Taipei Pediatrics, Department of Pediatrics, Chang Gung Children's Hospital, Chang Gung Memorial Hospital, Taoyuan, Taiwan; Department of Respiratory Therapy, Chang Gung Memorial Hospital, Taoyuan, Taiwan; College of Medicine, Chang Gung University, Taoyuan, Taiwan.
³ Graduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan.
⁴ School of Nursing, Chang Gung University, Taoyuan, Taiwan.
⁵ Department of Public Health, College of Medicine, Fu Jen Catholic University, New Taipei City, Taiwan. Electronic address: 056416@mail.fju.edu.tw.

PMID: 24491995
DOI: 10.1016/j.jfma.2013.12.009

Abstract

This study aimed to evaluate the feasibility of the chemical method to analyze exhaled breath condensate (EBC) leukotriene B4 (LTB4) level in humans. High-performance liquid chromatography with a UV detector was applied to quantify the inflammatory biomarker. The LTB4 concentration in the concentrated pooled EBC samples was 1.19 ng/μL, and the average LTB4 concentration of each EBC sample was 15.38 ng/μL. This analytical technique was feasible to evaluate the levels of inflammatory mediators such as LTB4 in human EBCs without any complicated sample pretreatment processes.

Keywords: exhaled breath condensate; high-performance liquid chromatography; leukotriene B(4); leukotrienes.

Publication types

Evaluation Study

MeSH terms

Adult
Biomarkers / analysis
Breath Tests
Chromatography, High Pressure Liquid / methods*
Exhalation
Female
Humans
Leukotriene B4 / analysis*
Male
Young Adult

Substances

Biomarkers
Leukotriene B4