Abstract
Pertussis toxin (PTx) has various effects in experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis (MS). This study was designed to explore the protective effects of PTx of different doses and subunits. EAE model was induced with myelin oligodendrocyte glycoprotein (MOG35-55, 200 ug) plus complete Freund's adjuvant in 6-7 week-old female C57BL/6 mice. PTx reduced clinical deficits of EAE by 91.3%. This reduction in clinical deficits was achieved by attenuating demyelination by 75.5%. Furthermore, PTx reduced the lymphocyte infiltration, deactivated microglia activation and changed T cell profile by increasing T helper (type 1 and 2) and T regulatory cells.
Keywords:
Experimental autoimmune encephalomyelitis; Microglia; Multiple sclerosis; Myelin oligodendrocyte glycoprotein; Pertussis toxin.
Copyright © 2014 Elsevier Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Analysis of Variance
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Animals
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Antigen Presentation / drug effects
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Calcium-Binding Proteins / metabolism
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Cell Proliferation / drug effects
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Cytokines / metabolism
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Disease Models, Animal
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Dose-Response Relationship, Drug
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Encephalomyelitis, Autoimmune, Experimental / chemically induced
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Encephalomyelitis, Autoimmune, Experimental / prevention & control*
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Flow Cytometry
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Forkhead Transcription Factors / metabolism
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Mice
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Mice, Inbred C57BL
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Microfilament Proteins / metabolism
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Microglia / drug effects*
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Myelin-Oligodendrocyte Glycoprotein / toxicity
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Neurologic Examination
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Peptide Fragments / toxicity
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Pertussis Toxin / pharmacology
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Pertussis Toxin / therapeutic use*
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Spleen / pathology
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T-Lymphocytes / drug effects*
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Time Factors
Substances
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Aif1 protein, mouse
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Calcium-Binding Proteins
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Cytokines
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Forkhead Transcription Factors
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Foxp3 protein, mouse
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Microfilament Proteins
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Myelin-Oligodendrocyte Glycoprotein
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Peptide Fragments
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myelin oligodendrocyte glycoprotein (35-55)
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Pertussis Toxin