This study investigated the influence of the regression model and initial intensity during an incremental test on the relationship between the lactate threshold estimated by the maximal-deviation method and performance in elite-standard runners. Twenty-three well-trained runners completed a discontinuous incremental running test on a treadmill. Speed started at 9 km · h(-1) and increased by 1.5 km · h(-1) every 4 min until exhaustion, with a minute of recovery for blood collection. Lactate-speed data were fitted by exponential and polynomial models. The lactate threshold was determined for both models, using all the co-ordinates, excluding the first and excluding the first and second points. The exponential lactate threshold was greater than the polynomial equivalent in any co-ordinate condition (P < 0.001). For both models, the lactate threshold was lower when all co-ordinates were used than when the first and the first and second points were excluded (P < 0.001). 10-km race pace was correlated with both the exponential and polynomial lactate thresholds for all the co-ordinate conditions (r ≥ 0.75, P < 0.001 and r ≥ 0.56, P < 0.05, respectively). The results suggest that the exponential lactate threshold should be used instead of the polynomial equivalent since it is more strongly associated with performance and is independent of the initial intensity of the test.