Establishment of a rescue system for an autonomous Parvovirus mink enteritis virus

Daoli Yuan; Jigui Wang; Zhili Li; Yaping Mao; Jia-Zeng Sun; Ji Xi; Shuang Wang; Qiang Hou; Bao Yi; Weiquan Liu

doi:10.1016/j.virusres.2014.01.012

Establishment of a rescue system for an autonomous Parvovirus mink enteritis virus

Virus Res. 2014 Apr:183:1-5. doi: 10.1016/j.virusres.2014.01.012. Epub 2014 Jan 22.

Authors

Daoli Yuan¹, Jigui Wang¹, Zhili Li¹, Yaping Mao¹, Jia-Zeng Sun¹, Ji Xi¹, Shuang Wang¹, Qiang Hou¹, Bao Yi¹, Weiquan Liu²

Affiliations

¹ State Key Laboratory of Agrobiotechnology, Department of Biochemistry and Molecular Biology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
² State Key Laboratory of Agrobiotechnology, Department of Biochemistry and Molecular Biology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China. Electronic address: weiquan6273@126.com.

PMID: 24463297
DOI: 10.1016/j.virusres.2014.01.012

Abstract

Construction and characterization of a full-length infectious clone (pMEV) of mink enteritis virus are described. Feline kidney cells (F81) were transfected with pMEV containing an engineered BamHI site that served as a genetic marker. The rescued virus was indistinguishable from its parental virus. The availability of a MEV infectious clone will facilitate studies of viral replication and pathogenicity and will permit the elucidation of determinants of the host range of the parvovirus.

Keywords: Biological properties; Genetic marker; Infectious clone; Mink enteritis virus; Rescue virus; Reverse genetics.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cats
Cell Line
Mink enteritis virus / genetics
Mink enteritis virus / growth & development*
Reverse Genetics / methods*
Virology / methods*