Effect of L-amino acid oxidase from Calloselasma rhodosthoma snake venom on human neutrophils

Toxicon. 2014 Mar:80:27-37. doi: 10.1016/j.toxicon.2013.12.013. Epub 2014 Jan 21.

Abstract

The in vitro effects of LAAO, an l-amino acid oxidase isolated from Calloselasma rhodosthoma snake venom, on isolated human neutrophil function were investigated. LAAO showed no toxicity on neutrophils. At non-cytotoxic concentrations, LAAO induced the superoxide anion production by isolated human neutrophil. This toxin, in its native form, is also able to stimulate the production of hydrogen peroxide in neutrophils, suggesting that its primary structure is essential for stimulation the cell. Moreover, the incubation of LAAO and phenol red medium did not induce the production of hydrogen peroxide. Furthermore, LAAO was able to stimulate neutrophils to release proinflammatory mediators such as IL-8 and TNF-α as well as NETs liberation. Together, the data showed that the LAAO triggers relevant proinflammatory events. Particular regions of the molecule distinct from the LAAO catalytic site may be involved in the onset of inflammatory events.

Keywords: Cytokines; LAAO; NETs; Neutrophils; Reactive oxygen species; Snake venom.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Animals
  • Cell Survival / drug effects
  • Humans
  • Hydrogen Peroxide / metabolism
  • Interleukin-8 / metabolism
  • L-Amino Acid Oxidase / pharmacology*
  • Neutrophils / cytology*
  • Neutrophils / drug effects*
  • Reactive Oxygen Species / metabolism
  • Tumor Necrosis Factor-alpha / metabolism
  • Viper Venoms / pharmacology*
  • Viperidae
  • Young Adult

Substances

  • Interleukin-8
  • Reactive Oxygen Species
  • Tumor Necrosis Factor-alpha
  • Viper Venoms
  • Hydrogen Peroxide
  • L-Amino Acid Oxidase