Potential halophilic cellulases for in situ enzymatic saccharification of ionic liquids pretreated lignocelluloses

Bioresour Technol. 2014 Mar:155:177-81. doi: 10.1016/j.biortech.2013.12.101. Epub 2014 Jan 2.

Abstract

Ionic liquids (ILs) have been used as an alternative green solvent for lignocelluloses pretreatment. However, being a salt, ILs exhibit an inhibitory effect on cellulases activity, thus making the subsequent saccharification inefficient. The aim of the present study is to produce salt-tolerant cellulases, with the rationale that the enzyme also tolerant to the presence of ILs. The enzyme was produced from a locally isolated halophilic strain and was characterized and assessed for its tolerance to different types of ionic liquids. The results showed that halophilic cellulases produced from Aspergillus terreus UniMAP AA-6 exhibited higher tolerance to ILs and enhanced thermo stability in the presence of high saline conditions.

Keywords: Cellulases; Halophiles; Ionic liquids; Lignocelluloses; Saccharification.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aspergillus / enzymology*
  • Aspergillus / genetics
  • Cellulase / metabolism*
  • DNA Primers / genetics
  • Hydrolysis
  • Ionic Liquids / metabolism*
  • Lignin / metabolism*
  • Polymerase Chain Reaction
  • Salinity
  • Temperature

Substances

  • DNA Primers
  • Ionic Liquids
  • lignocellulose
  • Lignin
  • Cellulase