Knockdown of a novel G-protein pathway suppressor 2 (GPS2) leads to shrimp mortality by exuvial entrapment during ecdysis

Fish Shellfish Immunol. 2014 Mar;37(1):46-52. doi: 10.1016/j.fsi.2014.01.007. Epub 2014 Jan 13.

Abstract

A novel G-protein pathway suppressor 2 (GPS2) has been identified from hemocytes of the whiteleg shrimp Penaeus vannamei (Pv) and appears to play a role in ecdysis. The full-length of PvGPS2 cDNA consisted of a 1230-bp open reading frame, encoding 409 deduced amino acids with significant sequence homology to GPS2 sequences of crustaceans and insects. RT-PCR revealed that PvGPS2 was expressed in all P. vannamei tissues examined, but that expression was molt stage specific in eyestalk tissue. Relative expression was higher in the period before molting (i.e., intermolt and pre-molt stages) than in the post-molt stage. When double-stranded RNA (dsRNA)-mediated RNA interference was employed to inhibit PvGPS2 formation in shrimp, it led to significant mortality due to unsuccessful separation of new cuticle from old cuticle (exuvial entrapment) during ecdysis.

Keywords: G-protein pathway suppressor 2; Molting; Penaeus vannamei; RNAi; Shrimp.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • DNA Primers / genetics
  • GTP-Binding Proteins / genetics
  • GTP-Binding Proteins / metabolism*
  • Gene Knockdown Techniques
  • Genes, Suppressor*
  • Hemocytes / metabolism
  • Histological Techniques
  • Molecular Sequence Data
  • Molting / genetics*
  • Molting / physiology
  • Penaeidae / genetics*
  • Penaeidae / physiology
  • RNA Interference
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Analysis, DNA
  • Signal Transduction / genetics*

Substances

  • DNA Primers
  • GTP-Binding Proteins

Associated data

  • GENBANK/JX185300