The effect of β-glucan on formation and functionality of neutrophil extracellular traps in carp (Cyprinus carpio L.)

Graham Brogden; Tanja Krimmling; Mikołaj Adamek; Hassan Y Naim; Dieter Steinhagen; Maren von Köckritz-Blickwede

doi:10.1016/j.dci.2014.01.003

The effect of β-glucan on formation and functionality of neutrophil extracellular traps in carp (Cyprinus carpio L.)

Dev Comp Immunol. 2014 Jun;44(2):280-5. doi: 10.1016/j.dci.2014.01.003. Epub 2014 Jan 14.

Authors

Graham Brogden¹, Tanja Krimmling², Mikołaj Adamek³, Hassan Y Naim⁴, Dieter Steinhagen³, Maren von Köckritz-Blickwede⁵

Affiliations

¹ Fish Disease Research Unit, University of Veterinary Medicine Hannover, Bünteweg 17, D-30559 Hannover, Germany; Department of Physiological Chemistry, University of Veterinary Medicine Hannover, Bünteweg 17, D-30559 Hannover, Germany.
² Fish Disease Research Unit, University of Veterinary Medicine Hannover, Bünteweg 17, D-30559 Hannover, Germany; Department of Physiological Chemistry, University of Veterinary Medicine Hannover, Bünteweg 17, D-30559 Hannover, Germany; Cell Biology and Immunology Group, Wageningen Institute of Animal Sciences, Wageningen University, PO Box 338, 6700 AH Wageningen, The Netherlands.
³ Fish Disease Research Unit, University of Veterinary Medicine Hannover, Bünteweg 17, D-30559 Hannover, Germany.
⁴ Department of Physiological Chemistry, University of Veterinary Medicine Hannover, Bünteweg 17, D-30559 Hannover, Germany.
⁵ Department of Physiological Chemistry, University of Veterinary Medicine Hannover, Bünteweg 17, D-30559 Hannover, Germany. Electronic address: mkoeckbl@tiho-hannover.de.

PMID: 24434196
DOI: 10.1016/j.dci.2014.01.003

Abstract

The formation of neutrophil extracellular traps (NETs) has been characterised as a novel antimicrobial host defence strategy of neutrophils besides phagocytosis and degranulation, which may lead to entrapment and subsequent immobilisation and/or killing of bacterial pathogens. Here we studied the effect of the feed additive β-glucan, namely MacroGard(®), on the formation and functionality of NETs in carp. Therefore, common carp (Cyprinus carpio) head kidney and kidney cells were isolated and treated with or without β-glucan over time. The formation of NETs was analysed by immunofluorescence microscopy and revealed a distinct increase of NET-formation with β-glucan. Furthermore the subsequent entrapment of Aeromonas hydrophila, an important fish pathogen, was increased after stimulating the cells with β-glucan. However, β-glucan did not lead to a stimulation of antimicrobial activity of neutrophils against A. hydrophila. In conclusion, the data underline the fact that the feed additive β-glucan is able to modulate carp neutrophil functions.

Keywords: Aeromonas hydrophila; Carp; Neutrophil extracellular traps (NETs); Neutrophils; β-Glucan.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aeromonas hydrophila / immunology*
Animals
Carps / immunology*
Dietary Supplements
Extracellular Space
Gene Expression Profiling
Gram-Negative Bacterial Infections / immunology*
Head Kidney / cytology*
Immunity, Innate / drug effects
Neutrophils / drug effects*
Neutrophils / immunology
beta-Glucans / pharmacology*

Substances

beta-Glucans