Bacteria adherent on the active or inactive surface can form biofilms in which they are encased in a hydrated matrix of extracellular polymeric substances, which is so called bacterial biofilm (BBF). BBF has greater virulence and drug resistance, they can also evade the host immune system and lead to immune injury compared with planktonic bacteria. Many common medical infections involving ophthalmic infection are biofilm-associated. In this paper, the concept and biological characteristics of BBF and BBF-related ophthalmic infection were reviewed.