Abstract
We characterized the relative fitness of multiple nonnucleoside reverse transcriptase (RT) inhibitor (NNRTI)-resistant HIV-1 variants in the presence of etravirine (ETV), rilpivirine (RPV), and/or the nucleoside RT inhibitor emtricitabine (FTC) by simultaneous competitive culture and 454 deep sequencing. The E138A substitution, typically associated with decreased virologic responses to ETV- and RPV-containing regimens, confers a clear fitness advantage to the virus in the presence of FTC and decreases FTC susceptibility 4.7-fold.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-HIV Agents / pharmacology*
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Deoxycytidine / analogs & derivatives*
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Deoxycytidine / pharmacology
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Drug Resistance, Viral / genetics
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Emtricitabine
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HIV Reverse Transcriptase / genetics*
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HIV Reverse Transcriptase / metabolism*
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Humans
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Virus Replication / drug effects
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Virus Replication / genetics
Substances
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Anti-HIV Agents
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Deoxycytidine
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reverse transcriptase, Human immunodeficiency virus 1
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HIV Reverse Transcriptase
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Emtricitabine