Molecular cytogenetics (FISH and fluorochrome banding): resolving species relationships and genome organization

Sonja Siljak-Yakovlev; Fatima Pustahija; Vedrana Vicic; Odile Robin

doi:10.1007/978-1-62703-767-9_15

Molecular cytogenetics (FISH and fluorochrome banding): resolving species relationships and genome organization

Methods Mol Biol. 2014:1115:309-23. doi: 10.1007/978-1-62703-767-9_15.

Authors

Sonja Siljak-Yakovlev¹, Fatima Pustahija, Vedrana Vicic, Odile Robin

Affiliation

¹ Laboratory Ecology, Systematic and Evolution, UMR 8079, CNRS-UPS-AgroParisTech, University Paris-Sud, Orsay, France.

PMID: 24415481
DOI: 10.1007/978-1-62703-767-9_15

Abstract

Fluorochrome banding (chromomycin, Hoechst, and DAPI) and fluorescence in situ hybridization (FISH) are excellent molecular cytogenetic tools providing various possibilities in the study of chromosomal evolution and genome organization. The constitutive heterochromatin and rRNA genes are the most widely used FISH markers. The rDNA is organized into two distinct gene families (18S-5.8S-26S and 5S) whose number and location vary within the complex of closely related species. Therefore, they are widely used as chromosomal landmarks to provide valuable evidence concerning genome evolution at chromosomal levels.

MeSH terms

Chromomycins / metabolism
Chromosome Banding / methods*
In Situ Hybridization, Fluorescence / methods*
Phylogeny*
Plant Roots / cytology
Plant Roots / genetics
Tissue Fixation

Substances

Chromomycins